Changes in freezing tolerance in hybrid poplar caused by up- and down-regulation of PtFAD2 gene expression.
Identifieur interne : 003353 ( Main/Exploration ); précédent : 003352; suivant : 003354Changes in freezing tolerance in hybrid poplar caused by up- and down-regulation of PtFAD2 gene expression.
Auteurs : Zhou Zhou [République populaire de Chine] ; Min-Jie Wang ; Shu-Tang Zhao ; Jian-Jun Hu ; Meng-Zhu LuSource :
- Transgenic research [ 1573-9368 ] ; 2010.
Descripteurs français
- KwdFr :
- Acides gras (analyse), Acides gras (métabolisme), Adaptation physiologique (génétique), Chimère (génétique), Chimère (métabolisme), Chimère (physiologie), Congélation (effets indésirables), Fatty acid desaturases (génétique), Fatty acid desaturases (métabolisme), Feuilles de plante (composition chimique), Feuilles de plante (métabolisme), Gènes de plante (MeSH), Métabolisme lipidique (génétique), Métabolisme lipidique (physiologie), Populus (enzymologie), Populus (génétique), Populus (métabolisme), Populus (physiologie), Régulation de l'expression des gènes codant pour des enzymes (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Régulation négative (génétique), Régulation négative (physiologie), Régulation positive (génétique), Régulation positive (physiologie), Transformation génétique (physiologie), Végétaux génétiquement modifiés (MeSH).
- MESH :
- analyse : Acides gras.
- composition chimique : Feuilles de plante.
- effets indésirables : Congélation.
- enzymologie : Populus.
- génétique : Adaptation physiologique, Chimère, Fatty acid desaturases, Métabolisme lipidique, Populus, Régulation négative, Régulation positive.
- métabolisme : Acides gras, Chimère, Fatty acid desaturases, Feuilles de plante, Populus.
- physiologie : Chimère, Métabolisme lipidique, Populus, Régulation négative, Régulation positive, Transformation génétique.
- Gènes de plante, Régulation de l'expression des gènes codant pour des enzymes, Régulation de l'expression des gènes végétaux, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Adaptation, Physiological (genetics), Chimera (genetics), Chimera (metabolism), Chimera (physiology), Down-Regulation (genetics), Down-Regulation (physiology), Fatty Acid Desaturases (genetics), Fatty Acid Desaturases (metabolism), Fatty Acids (analysis), Fatty Acids (metabolism), Freezing (adverse effects), Gene Expression Regulation, Enzymologic (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Lipid Metabolism (genetics), Lipid Metabolism (physiology), Plant Leaves (chemistry), Plant Leaves (metabolism), Plants, Genetically Modified (MeSH), Populus (enzymology), Populus (genetics), Populus (metabolism), Populus (physiology), Transformation, Genetic (physiology), Up-Regulation (genetics), Up-Regulation (physiology).
- MESH :
- chemical , analysis : Fatty Acids.
- chemical , genetics : Fatty Acid Desaturases.
- adverse effects : Freezing.
- chemistry : Plant Leaves.
- enzymology : Populus.
- genetics : Adaptation, Physiological, Chimera, Down-Regulation, Lipid Metabolism, Populus, Up-Regulation.
- metabolism : Chimera, Fatty Acid Desaturases, Fatty Acids, Plant Leaves, Populus.
- physiology : Chimera, Down-Regulation, Lipid Metabolism, Populus, Transformation, Genetic, Up-Regulation.
- Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Genes, Plant, Plants, Genetically Modified.
Abstract
In plant species, the level of polyunsaturated fatty acids (PUFAs) is essential for cold acclimation. To test whether changes in PUFA levels can lead to the alteration of freezing tolerance in poplar trees, we up- and down-regulated a Populus tomentosa Delta-12 fatty acid desaturase gene (PtFAD2) in the hybrid poplar (P. alba x P. glandulosa) clone 84 K. Real-time PCR results demonstrated that compared to untransformed control lines, the transcriptional level of PtFAD2 increased by up to 90% in over-expressing poplar lines (line OE-1) and decreased in down-regulated RNAi lines by up to 64% (line DR-1). As a result, the content of linoleic (C18:2) and linolenic (C18:3) unsaturated fatty acids (FAs) in total FAs increased by 7.5 and 3.9%, respectively, in the OE-1 line and decreased by 14.4 and 5.4% in the DR-2 line when compared to non-transgenic lines. After freezing treatment at -4 degrees C for 3 h without pre-cold acclimation, the survival rates of the PtFAD2-over-expressing cuttings were significantly higher (60% for OE-1) than those of non-transgenic plants (36.7%) and down-regulated lines (10% for DR-2). These results clearly demonstrate that the expression level of PUFAs substantially affected the freezing tolerance of hybrid poplar cuttings and could thus be utilized as an effective strategy to improve poplar anti-freezing traits through genetic engineering biotechnology.
DOI: 10.1007/s11248-009-9349-x
PubMed: 20012191
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Changes in freezing tolerance in hybrid poplar caused by up- and down-regulation of PtFAD2 gene expression.</title><author><name sortKey="Zhou, Zhou" sort="Zhou, Zhou" uniqKey="Zhou Z" first="Zhou" last="Zhou">Zhou Zhou</name><affiliation wicri:level="1"><nlm:affiliation>Lab of Biotechnology, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Lab of Biotechnology, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091</wicri:regionArea><wicri:noRegion>100091</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Min Jie" sort="Wang, Min Jie" uniqKey="Wang M" first="Min-Jie" last="Wang">Min-Jie Wang</name></author><author><name sortKey="Zhao, Shu Tang" sort="Zhao, Shu Tang" uniqKey="Zhao S" first="Shu-Tang" last="Zhao">Shu-Tang Zhao</name></author><author><name sortKey="Hu, Jian Jun" sort="Hu, Jian Jun" uniqKey="Hu J" first="Jian-Jun" last="Hu">Jian-Jun Hu</name></author><author><name sortKey="Lu, Meng Zhu" sort="Lu, Meng Zhu" uniqKey="Lu M" first="Meng-Zhu" last="Lu">Meng-Zhu Lu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20012191</idno><idno type="pmid">20012191</idno><idno type="doi">10.1007/s11248-009-9349-x</idno><idno type="wicri:Area/Main/Corpus">003361</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003361</idno><idno type="wicri:Area/Main/Curation">003361</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003361</idno><idno type="wicri:Area/Main/Exploration">003361</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Changes in freezing tolerance in hybrid poplar caused by up- and down-regulation of PtFAD2 gene expression.</title><author><name sortKey="Zhou, Zhou" sort="Zhou, Zhou" uniqKey="Zhou Z" first="Zhou" last="Zhou">Zhou Zhou</name><affiliation wicri:level="1"><nlm:affiliation>Lab of Biotechnology, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Lab of Biotechnology, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091</wicri:regionArea><wicri:noRegion>100091</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Min Jie" sort="Wang, Min Jie" uniqKey="Wang M" first="Min-Jie" last="Wang">Min-Jie Wang</name></author><author><name sortKey="Zhao, Shu Tang" sort="Zhao, Shu Tang" uniqKey="Zhao S" first="Shu-Tang" last="Zhao">Shu-Tang Zhao</name></author><author><name sortKey="Hu, Jian Jun" sort="Hu, Jian Jun" uniqKey="Hu J" first="Jian-Jun" last="Hu">Jian-Jun Hu</name></author><author><name sortKey="Lu, Meng Zhu" sort="Lu, Meng Zhu" uniqKey="Lu M" first="Meng-Zhu" last="Lu">Meng-Zhu Lu</name></author></analytic><series><title level="j">Transgenic research</title><idno type="eISSN">1573-9368</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptation, Physiological (genetics)</term><term>Chimera (genetics)</term><term>Chimera (metabolism)</term><term>Chimera (physiology)</term><term>Down-Regulation (genetics)</term><term>Down-Regulation (physiology)</term><term>Fatty Acid Desaturases (genetics)</term><term>Fatty Acid Desaturases (metabolism)</term><term>Fatty Acids (analysis)</term><term>Fatty Acids (metabolism)</term><term>Freezing (adverse effects)</term><term>Gene Expression Regulation, Enzymologic (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Lipid Metabolism (genetics)</term><term>Lipid Metabolism (physiology)</term><term>Plant Leaves (chemistry)</term><term>Plant Leaves (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Populus (physiology)</term><term>Transformation, Genetic (physiology)</term><term>Up-Regulation (genetics)</term><term>Up-Regulation (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides gras (analyse)</term><term>Acides gras (métabolisme)</term><term>Adaptation physiologique (génétique)</term><term>Chimère (génétique)</term><term>Chimère (métabolisme)</term><term>Chimère (physiologie)</term><term>Congélation (effets indésirables)</term><term>Fatty acid desaturases (génétique)</term><term>Fatty acid desaturases (métabolisme)</term><term>Feuilles de plante (composition chimique)</term><term>Feuilles de plante (métabolisme)</term><term>Gènes de plante (MeSH)</term><term>Métabolisme lipidique (génétique)</term><term>Métabolisme lipidique (physiologie)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Populus (physiologie)</term><term>Régulation de l'expression des gènes codant pour des enzymes (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Régulation négative (génétique)</term><term>Régulation négative (physiologie)</term><term>Régulation positive (génétique)</term><term>Régulation positive (physiologie)</term><term>Transformation génétique (physiologie)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Fatty Acids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fatty Acid Desaturases</term></keywords><keywords scheme="MESH" qualifier="adverse effects" xml:lang="en"><term>Freezing</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Acides gras</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Feuilles de plante</term></keywords><keywords scheme="MESH" qualifier="effets indésirables" xml:lang="fr"><term>Congélation</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Adaptation, Physiological</term><term>Chimera</term><term>Down-Regulation</term><term>Lipid Metabolism</term><term>Populus</term><term>Up-Regulation</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Adaptation physiologique</term><term>Chimère</term><term>Fatty acid desaturases</term><term>Métabolisme lipidique</term><term>Populus</term><term>Régulation négative</term><term>Régulation positive</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Chimera</term><term>Fatty Acid Desaturases</term><term>Fatty Acids</term><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides gras</term><term>Chimère</term><term>Fatty acid desaturases</term><term>Feuilles de plante</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Chimère</term><term>Métabolisme lipidique</term><term>Populus</term><term>Régulation négative</term><term>Régulation positive</term><term>Transformation génétique</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Chimera</term><term>Down-Regulation</term><term>Lipid Metabolism</term><term>Populus</term><term>Transformation, Genetic</term><term>Up-Regulation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Enzymologic</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Gènes de plante</term><term>Régulation de l'expression des gènes codant pour des enzymes</term><term>Régulation de l'expression des gènes végétaux</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In plant species, the level of polyunsaturated fatty acids (PUFAs) is essential for cold acclimation. To test whether changes in PUFA levels can lead to the alteration of freezing tolerance in poplar trees, we up- and down-regulated a Populus tomentosa Delta-12 fatty acid desaturase gene (PtFAD2) in the hybrid poplar (P. alba x P. glandulosa) clone 84 K. Real-time PCR results demonstrated that compared to untransformed control lines, the transcriptional level of PtFAD2 increased by up to 90% in over-expressing poplar lines (line OE-1) and decreased in down-regulated RNAi lines by up to 64% (line DR-1). As a result, the content of linoleic (C18:2) and linolenic (C18:3) unsaturated fatty acids (FAs) in total FAs increased by 7.5 and 3.9%, respectively, in the OE-1 line and decreased by 14.4 and 5.4% in the DR-2 line when compared to non-transgenic lines. After freezing treatment at -4 degrees C for 3 h without pre-cold acclimation, the survival rates of the PtFAD2-over-expressing cuttings were significantly higher (60% for OE-1) than those of non-transgenic plants (36.7%) and down-regulated lines (10% for DR-2). These results clearly demonstrate that the expression level of PUFAs substantially affected the freezing tolerance of hybrid poplar cuttings and could thus be utilized as an effective strategy to improve poplar anti-freezing traits through genetic engineering biotechnology.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20012191</PMID><DateCompleted><Year>2010</Year><Month>12</Month><Day>02</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-9368</ISSN><JournalIssue CitedMedium="Internet"><Volume>19</Volume><Issue>4</Issue><PubDate><Year>2010</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Transgenic research</Title><ISOAbbreviation>Transgenic Res</ISOAbbreviation></Journal><ArticleTitle>Changes in freezing tolerance in hybrid poplar caused by up- and down-regulation of PtFAD2 gene expression.</ArticleTitle><Pagination><MedlinePgn>647-54</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11248-009-9349-x</ELocationID><Abstract><AbstractText>In plant species, the level of polyunsaturated fatty acids (PUFAs) is essential for cold acclimation. To test whether changes in PUFA levels can lead to the alteration of freezing tolerance in poplar trees, we up- and down-regulated a Populus tomentosa Delta-12 fatty acid desaturase gene (PtFAD2) in the hybrid poplar (P. alba x P. glandulosa) clone 84 K. Real-time PCR results demonstrated that compared to untransformed control lines, the transcriptional level of PtFAD2 increased by up to 90% in over-expressing poplar lines (line OE-1) and decreased in down-regulated RNAi lines by up to 64% (line DR-1). As a result, the content of linoleic (C18:2) and linolenic (C18:3) unsaturated fatty acids (FAs) in total FAs increased by 7.5 and 3.9%, respectively, in the OE-1 line and decreased by 14.4 and 5.4% in the DR-2 line when compared to non-transgenic lines. After freezing treatment at -4 degrees C for 3 h without pre-cold acclimation, the survival rates of the PtFAD2-over-expressing cuttings were significantly higher (60% for OE-1) than those of non-transgenic plants (36.7%) and down-regulated lines (10% for DR-2). These results clearly demonstrate that the expression level of PUFAs substantially affected the freezing tolerance of hybrid poplar cuttings and could thus be utilized as an effective strategy to improve poplar anti-freezing traits through genetic engineering biotechnology.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Zhou</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Lab of Biotechnology, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Min-Jie</ForeName><Initials>MJ</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Shu-Tang</ForeName><Initials>ST</Initials></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Jian-Jun</ForeName><Initials>JJ</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Meng-Zhu</ForeName><Initials>MZ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>12</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Transgenic Res</MedlineTA><NlmUniqueID>9209120</NlmUniqueID><ISSNLinking>0962-8819</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005227">Fatty Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.19.-</RegistryNumber><NameOfSubstance UI="D044943">Fatty Acid Desaturases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.99.-</RegistryNumber><NameOfSubstance UI="C040448">delta-12 fatty acid desaturase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000222" MajorTopicYN="N">Adaptation, Physiological</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002678" MajorTopicYN="Y">Chimera</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015536" MajorTopicYN="N">Down-Regulation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D044943" MajorTopicYN="N">Fatty Acid Desaturases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005227" MajorTopicYN="N">Fatty Acids</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005615" MajorTopicYN="N">Freezing</DescriptorName><QualifierName UI="Q000009" MajorTopicYN="Y">adverse effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015971" MajorTopicYN="N">Gene Expression Regulation, Enzymologic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050356" MajorTopicYN="N">Lipid Metabolism</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015854" MajorTopicYN="N">Up-Regulation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>05</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>11</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>12</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20012191</ArticleId><ArticleId IdType="doi">10.1007/s11248-009-9349-x</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Biochim Biophys Acta. 1997 Sep 4;1348(1-2):10-6</Citation><ArticleIdList><ArticleId IdType="pubmed">9370311</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2003 Apr;44(4):447-50</Citation><ArticleIdList><ArticleId IdType="pubmed">12721386</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2001 Oct;57(1-2):34-42</Citation><ArticleIdList><ArticleId IdType="pubmed">11693931</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2006;57(4):897-909</Citation><ArticleIdList><ArticleId IdType="pubmed">16473891</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 1998 Oct 2;1394(1):3-15</Citation><ArticleIdList><ArticleId IdType="pubmed">9767077</ArticleId></ArticleIdList></Reference><Reference><Citation>Prog Lipid Res. 2002 May;41(3):254-78</Citation><ArticleIdList><ArticleId IdType="pubmed">11814526</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1985 Mar;77(3):740-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16664127</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1992 Jan 25;267(3):1502-9</Citation><ArticleIdList><ArticleId IdType="pubmed">1730697</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Cell Biol. 1996 Apr;6(4):148-53</Citation><ArticleIdList><ArticleId IdType="pubmed">15157478</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 1992 Oct;11(3):241-54</Citation><ArticleIdList><ArticleId IdType="pubmed">14969949</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1997 Jun;114(2):467-474</Citation><ArticleIdList><ArticleId IdType="pubmed">12223720</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2004 Nov 3;1666(1-2):142-57</Citation><ArticleIdList><ArticleId IdType="pubmed">15519313</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2001 Jan;125(1):89-93</Citation><ArticleIdList><ArticleId IdType="pubmed">11154304</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:541-568</Citation><ArticleIdList><ArticleId IdType="pubmed">15012300</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2006 Apr;223(5):1090-100</Citation><ArticleIdList><ArticleId IdType="pubmed">16292565</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 1996 Aug;14(8):1003-6</Citation><ArticleIdList><ArticleId IdType="pubmed">9631040</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1994 Jun;105(2):601-605</Citation><ArticleIdList><ArticleId IdType="pubmed">12232227</ArticleId></ArticleIdList></Reference><Reference><Citation>Prog Lipid Res. 2003 Nov;42(6):527-43</Citation><ArticleIdList><ArticleId IdType="pubmed">14559070</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2003 Feb;54(383):835-44</Citation><ArticleIdList><ArticleId IdType="pubmed">12554726</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Hu, Jian Jun" sort="Hu, Jian Jun" uniqKey="Hu J" first="Jian-Jun" last="Hu">Jian-Jun Hu</name><name sortKey="Lu, Meng Zhu" sort="Lu, Meng Zhu" uniqKey="Lu M" first="Meng-Zhu" last="Lu">Meng-Zhu Lu</name><name sortKey="Wang, Min Jie" sort="Wang, Min Jie" uniqKey="Wang M" first="Min-Jie" last="Wang">Min-Jie Wang</name><name sortKey="Zhao, Shu Tang" sort="Zhao, Shu Tang" uniqKey="Zhao S" first="Shu-Tang" last="Zhao">Shu-Tang Zhao</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Zhou, Zhou" sort="Zhou, Zhou" uniqKey="Zhou Z" first="Zhou" last="Zhou">Zhou Zhou</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003353 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003353 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:20012191
|texte= Changes in freezing tolerance in hybrid poplar caused by up- and down-regulation of PtFAD2 gene expression.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20012191" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |